About Ifosfamide Alkylating Agent (Nitrogen Mustard), Antineoplastic Mechanism of Action of Ifosfamide In the liver drug undergo enzymatic activation into 4-hydroxyifosfamide.This alkylates the DNA by binding to N7 position of the guanine residue. This results cross linking/abnormal base pairing/scission of DNA strand. There is cross linking of nucleic acid with proteins can also takes place. The overall process is cell cycle nonspecific reaction. Indications for Ifosfamide 1. Testicular cancer 2. Breast cancer 3. Non-Hodgkins disease 4. Hodgkins disease 5. Soft tissue sarcoma 6. Osteogenic sarcoma 7. Lung cancer 8. Cervical cancer 9. Ovarian cancer 10. Lymphocytic lymphoma 11. Gastric cancer 12. Pancreatic cancer 13. Bladder cancer 14. Cancer of endometrium 15. Neuroblastoma 16. Wilm`s tumor